Volume 7, Issue 3 (Aug 2022)                   JNFS 2022, 7(3): 299-310 | Back to browse issues page

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Salehian Y, Sobhanipour A, Mohammadi-Moghadam M, Fani S R. Contamination of Stored Pistachios to Aspergillus Section Flavi and Aflatoxins. JNFS 2022; 7 (3) :299-310
URL: http://jnfs.ssu.ac.ir/article-1-422-en.html
Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran.
Abstract:   (1193 Views)
Background: Contamination of stored pistachio nuts to Aspergillus section Flavi and aflatoxins (AFs) as well as effective parameters on fungal growth and AF production during storage was assayed. Methods: Twenty one pistachio nut samples were taken from some warehouse of Damghan county. Different parameters of storage, including temperature, relative humidity, and pest infestation were recorded. Contamination to aflatoxigenic fungi was investigated by serial dilution method and aspergillus flavus and parasiticus agar (AFPA) medium with three repetitions. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) method were used to measure quality and quantity of the produced AFs, respectively. Results: There was a significant difference in contamination to Aspergillus section Flavi between different samples on the basis of statistical analysis. There was no significant difference between samples contamination to Aspergillus section Flavi and storage parameters. The ability of AF production of isolates was variable. More than 88% of isolates were able to produce one or several types of AFs and less than 12% of isolates produced no AF. HPLC assay showed that out of 21 pistachio nut samples, 3 samples were contaminated with various types of AFs, but were below the allowable levels (P ≤ 0.05). Conclusion: Despite the population of aflatoxigenic strains in the mycoflora of warehouse, AF contamination of pistachios was estimated to be about 14%, which was also less than the allowable level. The results showed that the environmental parameters required for the production of AF in the warehouse of Semnan province do not exist and the storage conditions in this province are suitable.
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Type of article: orginal article | Subject: public specific
Received: 2021/06/8 | Published: 2022/08/19 | ePublished: 2022/08/19

1. Azizi R, Ghosta Y & Ahmadpour A 2020. New fungal canker pathogens of apple trees in Iran. Journal of crop protection. 9 (4): 669-681.
2. Bandyopadhyay R, et al. 2016. Biological control of aflatoxins in Africa: current status and potential challenges in the face of climate change. World mycotoxin journal. 9 (5): 771-789.
3. Benkerroum N 2019. Retrospective and prospective look at aflatoxin research and development from a practical standpoint. International journal of environmental research and public health. 16 (19): 3633.
4. Bennett J, Kale S & Yu J 2007. Aflatoxins: background, toxicology, and molecular biology. In Foodborne diseases, pp. 355-373. Springer.
5. Bensassi F, Rhouma A, Ghrab M, Bacha H & Hajlaoui MR 2010. Evaluation of cultivar susceptibility and storage periods towards aflatoxin B1 contamination on pistachio nuts. Mycotoxin research. 26 (3): 199-203.
6. Cotty P 1990. Effect of atoxigenic strains of Aspergillus flavus on aflatoxin contamination of developing cottonseed. Plant disease. 74 (3): 233-235.
7. Danesh D, Mojtahedi H, Barnett R & Campbell A 1979. Correlation between climatic data and aflatoxin contamination of Iranian pistachio nuts. Phytopathology. 69 (7): 715-716.
8. Ellis WO, Smith JP, Simpson BK, Ramaswamy H & Doyon G 1994. Growth of and aflatoxin production by Aspergillus flavus in peanuts stored under modified atmosphere packaging (MAP) conditions. International journal of food microbiology. 22 (2-3): 173-187.
9. Fallah A, Farhoodi M & Moradi V 2013. An Assessment on Aflatoxin Control in Pistachio‐Processing Units from Raw Material Reception to Packaging Based on ISO 22000: 2005 Model. Journal of food safety. 33 (4): 379-386.
10. Fani S, Moradi M, Zamanizadeh H, Mirabolfathy M & Probst C 2014a. Distribution of nontoxigenic strains of Aspergillus flavus throughout pistachio growing areas in Iran. Applied entomology and phytopathology. 81 (2): 179-190.
11. Fani SR, et al. 2014b. A critical evaluation of cultural methods for the identification of atoxigenic Aspergillus flavus isolates for aflatoxin mitigation in pistachio orchards of Iran. European journal of plant pathology. 140 (4): 631-642.
12. Frisvad JC, et al. 2019. Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins. Studies in mycology. 93: 1-63.
13. Georgiadou M, Dimou A & Yanniotis S 2012. Aflatoxin contamination in pistachio nuts: A farm to storage study. Food control. 26 (2): 580-586.
14. Ghadarijani M & Javanshah A 2005. Distribution of aflatoxin in processed pistachio nut terminals. In IV International Symposium on Pistachios and Almonds 726, pp. 431-436.
15. Gourama H & Bullerman LB 1995. Aspergillus flavus and Aspergillus parasiticus: Aflatoxigenic fungi of concern in foods and feeds: A review. Journal of food protection. 58 (12): 1395-1404.
16. Gupta A & Gopal M 2002. Aflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pests. World journal of microbiology and biotechnology. 18 (4): 329-335.
17. Marín S, Ramos AJ & Sanchis V 2012. Modelling Aspergillus flavus growth and aflatoxins production in pistachio nuts. Food microbiology. 32 (2): 378-388.
18. Mehrnezhad M & Panahi B 2006. The influence of hull cracking on aflatoxin contamination and insect infestation in pistachio nuts. Applied entomology and phytopatology. 76 (2): 105-123.
19. Moghadam MM, et al. 2020. The Potential of Aflatoxin Production in the Aspergillus Section Flavi Isolates of Pistachio in Iran. Journal of fasting & health. 8 (4).
20. Mojtahedi H, Danesh D, Haghighi B & Fathi S 1980. Storage relative humidity in Rafsanjan and impossibility of pistachio aflatoxicosis after nut processing. Iranian journal of plant pathology. 16 (1/4): 20-21.
21. Moradi M, et al. 2011. Screening of atoxigenic Aspergillus flavus isolates in pistachio producing areas of Iran. In International Symposium on Mycotoxins in Nuts and Dried Fruits 963, pp. 91-93.
22. Moradi M, Ershad D, Miraboufathy M & Panahi B 2004. The role of plant debris, soil and manure on population density of Aspergillus flavus and Aspergillus niger groups in pistachio orchards of Kerman province. Iranian journal of plant pathology. 40 (3-4): 221-234.
23. Moradi M, Fani SR & Masoumi H 2014. Population density of Aspergillus species belong to section Flavi and Nigri on pistachio nut in Kerman Province. Journal of applied researches in plant protection. 3 (2): 79-91.
24. Moradi M, HokmAbadi H & Fani S 2015. A Study Concerned with the Factors Affecting the Fungal Growth and Aflatoxin Production During Storage of Pistachio in Kerman Province. Journal of food technology and nutrition. 12 (2): 83-92.
25. Ostry V, Toman J, Grosse Y & Malir F 2018. Cyclopiazonic acid: 50th anniversary of its discovery. World mycotoxin journal. 11 (1): 135-148.
26. Pitt JI & Miller JD 2017. A concise history of mycotoxin research. Journal of agricultural and food chemistry. 65 (33): 7021-7033.
27. Pourhosseini R, et al. 2020. Potential of Cyclopiazonic Acid Production in Non-Aflatoxinogenic Strains of Aspergillus flavus of Iranian Pistachio. Pistachio and health journal. 3 (3): 6-17.
28. Set E & Erkmen O 2010. The aflatoxin contamination of ground red pepper and pistachio nuts sold in Turkey. Food and chemical toxicology. 48 (8-9): 2532-2537.
29. Sweeney RA & Rexroad PR 1987. Comparison of LECO FP-228" nitrogen determinator" with AOAC copper catalyst Kjeldahl method for crude protein. Journal of the association of official analytical chemists. 70 (6): 1028-1030.
30. Tavakolipour H, Basiri A & Kalbasl AA 2009. Effects of temperature and relative humidity on pistachio quality factors during storage time. Iranian journal of food science and technology. 5 (4): 57-66.
31. Wei D-L & Jong S-C 1986. Production of aflatoxins by strains of the Aspergillus flavus group maintained in ATCC. Mycopathologia. 93 (1): 19-24.

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