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Volume 9, Issue 4 (Nov 2024)
JNFS 2024, 9(4): 599-608 |
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Arab M, Naseri M, Shahi Sadr Abadi F, Nasri S. The Effect of Aerial Part of Melissa Officinalis L. Hydro-Alcoholic Extract on Pituitary- Gonadal Axis Function in Diabetic Male Mice. JNFS 2024; 9 (4) :599-608
URL: http://jnfs.ssu.ac.ir/article-1-844-en.html
URL: http://jnfs.ssu.ac.ir/article-1-844-en.html
Department of Biology, Payame Noor University, Tehran, Iran
Abstract: (710 Views)
Melissa officinalis L. ( lemon balm) is one of the more widely cultivated medicinal and aromatic plants that has long been used in traditional medicine to treat many disorders. The present study investigates Melissa officinalis L. hydro-alcoholic extract regarding pituitary-gonadal axis in diabetic mice. Methods: 45 NMRI mice with a mean weight of 35.6±4.5 g were divided into five groups: control group (0.2 ml of physiological serum intraperitoneally injection), diabetic mice (without treatment), and three experiment groups (diabetic groups receiving 50, 100 and 200 mg/kg of Melissa officinalis extract intraperitoneally injection). Melissa officinalis extract was injected intraperitoneally for 14 days. At the end of the experiment, blood samples were taken to determine the biochemical indicators level (glucose, LH, FSH, and testosterone), and the left testicle was weighed and examined histologically. Results: The results showed that the lowest amount of glucose and the highest level of LH were observed in the treatment group at a dose of 100 mg/kg of Melissa officinalis extract. The highest level of FSH was observed at a dose of 200 mg/kg. Histological study of the testis showed a more favorable condition in the experimental group of 200 mg/kg of lemon balm extract (P<0.05). Conclusion: It can be concluded that the use of Melissa officinalis extract is efficient in reducing glucose, improving the levels of LH, FSH, and testosterone in diabetic mice, affecting testicular weight, and improving testicular tissue indices and reproductive function of rats.
Keywords: Melissa officinalis, Diabetes mellitus, Gonadotropin hormones, Testosterone, Spermatogenesis, mice
Type of article: orginal article |
Subject:
public specific
Received: 2023/03/7 | Published: 2024/11/20 | ePublished: 2024/11/20
Received: 2023/03/7 | Published: 2024/11/20 | ePublished: 2024/11/20
References
1. Abbasi MM, Dadkhah N, Shahnazi M & Parvin N 2016. Protective Effects of Melissa officinalis (Lemon Balm) on Sperm Parameters and Spermatogenesis Quality in Rats Exposed to Lead. Iranian Red Crescent Medical Journal. 18 (12): 20-.
3. Abolfazl S, Amirhossein S & Behjat J 2016. Melissa officinalis L. – A review of its traditional uses, phytochemistry and pharmacology. Journal of ethnopharmacology. 188: 204-228.
4. Alijaniha F, et al. 2015. Heart palpitation relief with Melissa officinalis leaf extract: double blind, randomized, placebo controlled trial of efficacy and safety. Journal of ethnopharmacol. 164: 378-384.
5. Araj-Khodaei M, et al. 2020. A double-blind, randomized pilot study for comparison of Melissa officinalis L. and Lavandula angustifolia Mill. with Fluoxetine for the treatment of depression. BMC complementary medicine and therapies. 20 (1): 207.
6. Ashkani-Esfahani S, et al. 2021. The Effect of Melissa officinalis Extract on Streptozotocin-Induced Diabetes in Rats: A Stereological Study on Pancreatic Islets and Beta-cells. Journal of advances in medical and biomedical research. 29 (132): 34-40.
7. Babaeian M, et al. 2015. Herbal Remedies for Functional Dyspepsia and Traditional Iranian Medicine Perspective. Iran Red Crescent Medical Journal. 17 (11): e20741.
8. Ballester J, et al. 2004. Insulin-dependent diabetes affects testicular function by FSH-and LH-linked mechanisms. Journal of andrology. 25 (5): 706-719.
9. Behzadi A, et al. 2023. Antiviral Potential of Melissa officinalis L.: A Literature Review. Nutrition and metabolic insights. 16: 11786388221146683.
10. Chung MJ, Cho SY, Bhuiyan MJ, Kim KH & Lee SJ 2010. Anti-diabetic effects of lemon balm ( Melissa officinalis) essential oil on glucose- and lipid-regulating enzymes in type 2 diabetic mice. British journal of nutrition. 104 (2): 180-188.
11. Darvish-Mofrad-Kashani Z, et al. 2018. Effect of Melissa officinalis (Lemon balm) on Sexual Dysfunction in Women: A Double- blind, Randomized, Placebo-controlled Study. Iranian journal of pharmaceutical research. 17 (Suppl): 89-100.
12. Diogo P, et al. 2023. Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts. International journal of molecular sciences. 24 (8): 7035.
13. Draginic N, et al. 2021. Melissa officinalis L. as a Nutritional Strategy for Cardioprotection. Frontiers in physiology. 12.
14. Ghazizadeh J, et al. 2021. The effects of lemon balm (Melissa officinalis L.) on depression and anxiety in clinical trials: A systematic review and meta-analysis. Phytotherapy research. 35 (12): 6690-6705.
15. Guneli E, et al. 2008. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. European surgical research. 40 (4): 354-360.
16. Haybar H, et al. 2018. The effects of Melissa officinalis supplementation on depression, anxiety, stress, and sleep disorder in patients with chronic stable angina. Clinical nutrition ESPEN. 26: 47-52.
18. Hsiu-Fang Y, Chi-Ting H, Tusty-Jiuan H, Fang-Rong C & Chin-Kun W 2015. In vitro anti-diabetic effect and chemical component analysis of 29 essential oils products. Journal of food and drug analysis. 23 (1): 124-129.
19. Hussain T, et al. 2023. Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight. Frontiers in endocrinology. 14: 1070692.
20. Ighodaro OM, Adeosun AM & Akinloye OA 2017. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina 53 (6): 365-374.
21. Kato-Noguchi H 2003. Assessment of allelopathic potential of shoot powder of lemon balm. Scientia horticulturae. 97: 419-423.
22. Khaneshi F, Nasrolahi O, Azizi S & Nejati V 2013. Sesame effects on testicular damage in streptozotocin-induced diabetes rats. Avicenna journal of phytomedicine. 3 (4): 347-355.
23. Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D & Asadi-Samani M 2016. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron physician. 8 (1): 1832-1842.
24. Korejo NA, Wei QW, Shah AH & Shi FX 2016. Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals. Journal of Zhejiang University SCIENCE B. 17 (11): 850-863.
25. López V, et al. 2009. Neuroprotective and neurological properties of Melissa officinalis. Neurochemical research. 34 (11): 1955-1961.
26. Mallidis C, Agbaje I, O'Neill J & McClure N 2009. The influence of type 1 diabetes mellitus on spermatogenic gene expression. Fertility and Sterility. 92 (6): 2085-2087.
27. Mardanshahi T, Rezaei N, Zare Z, Malekzadeh Shafaroudi M & Mohammadi H 2019. Effects of L-Carnitine on the sperm parameters disorders, apoptosis of spermatogenic cells and testis histopathology in diabetic Rats. International journal of reproductive bioMedicine. 17 (5): 325-336.
28. Maresch CC, et al. 2017. Diabetes-induced hyperglycemia impairs male reproductive function: a systematic review. Human reproduction update. 24 (1): 86-105.
29. Mariarosaria L, et al. 2020. Impact of extraction processes on phytochemicals content and biological activity of Citrus × clementina Hort. Ex Tan. leaves: New opportunity for under-utilized food by-products. Food research international. 127: 108742.
30. Miraj S, Rafieian K & Kiani S 2017. Melissa officinalis L: A Review Study With an Antioxidant Prospective. Evidence-based complementary and alternative medicine. 22 (3): 385-394.
31. Moudi B, Heydari Z, Mahmoudzadeh Sagheb HR & Harati M 2007. Biochemical and Histological Study of Protective Effect of Sodium Tungstate on Oxidative Stress Induced by Streptozotocin in Pancreas of Diabetic Rats. Anatomical sciences journal. 5 (21): 0-0.
32. Moussa S 2008. Oxidative stress in diabetes Mellitus. Romanian journal of biophysics. 18: 225-236.
33. Naghdi M, et al. 2016. Effects of Common Fig (Ficus carica) Leaf Extracts on Sperm Parameters and Testis of Mice Intoxicated with Formaldehyde. Evidence-based complementary and alternative medicine. 2016: 2539127.
34. Naseri M, et al. 2021. The effect of Melissa officinalis L. extract on learning and memory: Involvement of hippocampal expression of nitric oxide synthase and brain-derived neurotrophic factor in diabetic rats. Journal of ethnopharmacology. 276: 114210.
35. Naseri M, et al. 2016. Bloating: Avicenna's Perspective and Modern Medicine. Evidence-based complementary and alternative medicine. 21 (2): 154-159.
36. Negahdary M, Arefian Z, Dastjerdi HA & Ajdary M 2015. Toxic effects of Mn2O3 nanoparticles on rat testis and sex hormone. Journal of natural science, biology and medicine. 6 (2): 335-339.
37. Noguchi-Shinohara M, et al. 2023. Effects of Melissa officinalis Extract Containing Rosmarinic Acid on Cognition in Older Adults Without Dementia: A Randomized Controlled Trial. Journal of Alzheimer's disease. 91 (2): 805-814.
38. Noori Roshnavand F, Hojati V, Vaezi G & Rahbarian R 2019. The Comparison of Green Tea Aqueous Extract and Catechin Effect on Pituitary-Gonadal Axis in Rat Models of Type 1 Diabetes. Journal of advances in medical and biomedical research. 27 (125): 9-15.
39. Petrisor G, et al. 2022. Melissa officinalis: Composition, Pharmacological Effects and Derived Release Systems-A Review. International journal of molecular sciences. 23 (7).
40. Rains JL & Jain SK 2011. Oxidative stress, insulin signaling, and diabetes. Free radical biology and medicine. 50 (5): 567-575.
41. Sadat SS, et al. 2019. Effects of Carob Fruit Extract on Spermatogenesis, Antioxidant Status, and Apoptosis in Adult Male Mice. Pharmaceutical sciences. 25 (3): 184-189.
42. Sadeghi N, Boissonneault G, Tavalaee M & Nasr-Esfahani MH 2023. Oxidative versus reductive stress: a delicate balance for sperm integrity. Systems biology in reproductive medicine. 69 (1): 20-31.
43. Saylam B & Çayan S 2020. Do antioxidants improve serum sex hormones and total motile sperm count in idiopathic infertile men? Turkish journal of urology. 46 (6): 442-448.
44. Schoeller EL, Schon S & Moley KH 2012. The effects of type 1 diabetes on the hypothalamic, pituitary and testes axis. Cell and tissue research 349 (3): 839-847.
45. Seif MM KF, Abou Arab AAK, Abdel-Aziz AS, Abou Donia MA. and Mohamed Sh. R 2014. Protective effect of Melissa officinalis L. against malathion toxicity and reproductive in male rats. International scholarly and scientific research & innovation 8:931-936.
46. Sheikhpour R 2013. Diabetes and Oxidative Stress: The Mechanism and Action. Iranian journal of diabetes and obesity. 5 (1): 40-45.
47. Shin Y, et al. 2021. The herbal extract ALS-L1023 from Melissa officinalis reduces weight gain, elevated glucose levels and β-cell loss in Otsuka Long-Evans Tokushima fatty rats. Journal of ethnopharmacol. 264: 113360.
48. Singh S, et al. 2006. Effects of streptozotocin-diabetes and insulin replacement on androgen and estrogen receptor concentrations in the epididymis of Wistar rats.
49. Standl E, Khunti K, Hansen TB & Schnell O 2019. The global epidemics of diabetes in the 21st century: Current situation and perspectives. European journal of preventive cardiology. 26 (2_suppl): 7-14.
50. Tang XY, et al. 2008. Effects of strontium fructose 1,6-diphosphate on expression of apoptosis-related genes and oxidative stress in testes of diabetic rats. International journal of urology. 15 (3): 251-256.
51. Uropean medicines agency 2013. Assessment report on Melissa officinalis L. folium.
52. Verma L, et al. 2010. Effect of ethanolic extract of Cassia occidentalis Linn. for the management of alloxan-induced diabetic rats. Pharmacognosy research. 2 (3): 132-137.
53. Weidner C, et al. 2014. Lemon balm extract causes potent antihyperglycemic and antihyperlipidemic effects in insulin-resistant obese mice. Molecular nutrition & food research. 58 (4): 903-907.
54. Yamasaki K, et al. 2022. Effects of antioxidant co-supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation-reduction potential levels in spermatozoa: A pilot study. Reproductive medicine and biology. 21 (1): e12450.
55. Zhong O, Ji L, Wang J, Lei X & Huang H 2021. Association of diabetes and obesity with sperm parameters and testosterone levels: a meta-analysis. Diabetology & metabolic syndrome. 13 (1): 109.
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