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Kimiaee M, Madani M, Mosaddegh M H, Moshtaghioun S M. The Study of Food Pathogens in Yazd Traditional Confectionary Products. JNFS 2024; 9 (2) :200-206
URL: http://jnfs.ssu.ac.ir/article-1-680-en.html
URL: http://jnfs.ssu.ac.ir/article-1-680-en.html
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Keywords: Food contamination, Microbial load, Fungal contamination, Traditional confectionery products, Yazd
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1 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran; 2 Shahid Sadoughi University of Medical Sciences, Pharmacy School, Yazd, Iran; 3 Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
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The Study of Food Pathogens in Yazd Traditional Confectionary Products
Mitra Kimiaee; MSc1, Mahboobeh Madani; PhD1*, Mohammad Hossein Mosaddegh; PhD2 &
Seyed Mohammad Moshtaghioun; PhD3
Mitra Kimiaee; MSc1, Mahboobeh Madani; PhD1*, Mohammad Hossein Mosaddegh; PhD2 &
Seyed Mohammad Moshtaghioun; PhD3
1 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran; 2 Shahid Sadoughi University of Medical Sciences, Pharmacy School, Yazd, Iran; 3 Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
| ARTICLE INFO | ABSTRACT | |
| ORIGINAL ARTICLE | Background: Food poisoning could be caused by eating the sweets contaminated with microorganisms, which has always been considered as one of the major problems of people in developing countries, including Iran. The aim of this study is to investigate microbial variety and fungal load in the traditional sweets offered in confectionery stores in Yazd province in 2020. Methods: In this study, 170 samples of traditional sweets, referred by confectioneries in Yazd province, were randomly selected and tested for microorganisms contamination such as Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, Salmonella, molds, and yeasts according to Iranian national standards. Results: The highest rate of microbial contamination in samples was related to "Pistachio Loz" (52.94%) and the lowest rate was related to "Hajibadam" (11.76%). The infection rates of Enterobacteriaceae, Escherichia coli, molds, and yeasts were 17.06%, 8.83%, 20.59%, 3.53%, respectively. No infection was observed with Staphylococcus aureus and Salmonella in the samples. Conclusions: Due to the high level of microbial contamination of traditional sweets, especially "Pistachio Loz" offered in Yazd, more control measures are needed in preparation and distribution of such sweets. Keywords: Food contamination; Microbial load; Fungal contamination; Traditional confectionery products; Yazd |
|
| Article history: Received:18 Jul 2022 Revised:22 Oct 2022 Accepted: 22 Oct 2022 |
||
| *Corresponding author mmadani66@gmail.com Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. Postal code: 8451731167 Tel: 98 09134097629 |
Introduction
Foods contain many pathogens (bacteria, viruses and parasites), which can be transmitted to humans by eating or drinking (Newell et al., 2010). Food-borne disease is one of the important causes of death in undeveloped countries. Symptoms could happen in a few minutes to a few weeks and include vomiting, diarrhea, fever, etc. (Bula-Rudas et al., 2015, Newell et al., 2010). Many types of microorganisms or toxins produced by them, cause food-borne diseases with different mechanisms (Mozafari et al., 2002). The US Centers for Disease Control and Prevention (CDC) estimated that 75 million people suffer from food-borne diseases each year (Newell et al., 2010, Ostadrahimi, 2010).
Since 1990, three major food-borne bacterial groups, including Salmonella, Escherichia coli, and Campylobacter had been studied in food industry (Meng and Doyle, 2002, Newell et al., 2010). Countries such as the United Kingdom and the United States, reported that 20-40% of illnesses were caused by eating contaminated food (Bolton et al., 2008). Contamination with mold and yeast may reduce the shelf life of confectionery products, causing unpleasant odors, tastes, and discoloration. Fungal contamination of confectionery raw materialsand confectionery containers and distributors could also lead to contamination of various types of sweets (Azhdari and Hashemzehi, 2020).
In developing countries such as Iran, the prevalence of food-borne infections is much higher than developed countries. Dairy, confectionery, and meat products in foods are the most common cause of food poisoning (Nikniaz et al., 2011). Traditional sweets, due to the type of ingredients, methods of preparation and decoration, are likely to be contaminated with pathogens such as Escherichia coli, Enterobacteriaceae, mold and yeast. Sweets have a variety of nutrients that are well known all over the world. Due to the high consumption of these products, it is necessary to use microbial control in order to increase shelf life and maintain the quality of these products (Smith et al., 2004).
The results of a study conducted by Sultan Dalal et al. on microbial contamination of fresh sweets in south of Tehran indicated that 72.7% of the sweets in the study were contaminated with Enterobacteriaceae (40%), yeasts (33%) and Staphylococcus aureus (12%), which were the highest types of microbes detected (Soltan Dalal et al., 2010). Another study in Gorgan investigated the contamination of sweets containing cream. The results confirmed that 56% of the samples were infected with Enterobacteriaceae and 43.3% were infected with Staphylococcus aureus, and the highest contamination of sweets was in summer (Shabani et al., 2014).
The study of microbial quality control regarding sweet samples in Leeds, England, showed that 28% of the total samples were infected with Escherichia coli (Azhdari and Hashemzehi, 2020). In evaluation of sweets containing cream and ice creams, 50% of the samples were infected with Escherichia coli and 30% with Staphylococcus aureus (Khoramrooz et al., 2015). Due to the increase in food poisoning caused by the consumption of contaminated sweets in recent years, few studies were performed in this regard. The present study was conducted to investigate the microbial and fungal variety and load in Yazd traditional confectionery products such as Baqlava, Quttab, Pashmak, Nan Berenji, Loz and Hajibadam. These products have different ingredients and moisture levels, which are definitely effective on the type and level of contamination.
Materials and Methods
This cross-sectional study was conducted in 2020 in Yazd, and chemicals and culture media used in this study were prepared and applied according to the Iranian National Standard numbers 2395 and 9899 from the German company (Merck) (Institute of Standards, 2020). Sample preparation and dilutions were performed using the Iranian National Standard No. 8923-1 and 8923-4. The traditional sweet samples were taken from Yazd province confectioneries. One hundred and seventy samples received by Food, Cosmetics and Hygiene Supervision Department of the Food and Drug Administration of Yazd Shahid Sadoughi University of Medical Sciences were used in this study. The methods used in microbiological experiments were in accordance with the Institute of Standards and Industrial Research of Iran standards of 10899-3, 1810, 6806-3, 2964, 2461-1, 2461-2 (Khoramrooz et al., 2015, Soltan Dalal et al., 2010). Microbial tests including Enterobacteriaceae count, Escherichia coli count, Staphylococcus aureus count, Salmonella count, mold and yeast count were evaluated.
Samples included various Yazd traditional confectionery products which were investigated as soon as possible after entering the laboratory. Five grams of samples were mixed with 45 ml of ringer solution, and they were cultured using specific culture media (for each type of microorganism). They were incubated at different temperatures (each sample was incubated for a certain period according to the type of bacteria tested). The samples were taken out afterwards, and their contamination was determined by national standard values (Institute of Standards, 2020). Samples with hihger than standard contamination were declared unusable, and samples without contamination or the ones with less than standard contamination were accepted (Table 1).
Enterobacteriaceae were counted according to the Iranian national standard numbers, 2461-2 and 2461-1. Escherichia coli was examined in accordance with the standard number 2946. Staphylococcus aureus coagulase positive test was performed according to the Iranian National Standard Number 6806-3. Salmonella was also examined according to the standard number 1810. Mold and yeast tests were performed according to the number of 10899-3.
Data analysis: Data were analyzed using SPSS 22 software and Chi-square and Fisher tests.
Since 1990, three major food-borne bacterial groups, including Salmonella, Escherichia coli, and Campylobacter had been studied in food industry (Meng and Doyle, 2002, Newell et al., 2010). Countries such as the United Kingdom and the United States, reported that 20-40% of illnesses were caused by eating contaminated food (Bolton et al., 2008). Contamination with mold and yeast may reduce the shelf life of confectionery products, causing unpleasant odors, tastes, and discoloration. Fungal contamination of confectionery raw materialsand confectionery containers and distributors could also lead to contamination of various types of sweets (Azhdari and Hashemzehi, 2020).
In developing countries such as Iran, the prevalence of food-borne infections is much higher than developed countries. Dairy, confectionery, and meat products in foods are the most common cause of food poisoning (Nikniaz et al., 2011). Traditional sweets, due to the type of ingredients, methods of preparation and decoration, are likely to be contaminated with pathogens such as Escherichia coli, Enterobacteriaceae, mold and yeast. Sweets have a variety of nutrients that are well known all over the world. Due to the high consumption of these products, it is necessary to use microbial control in order to increase shelf life and maintain the quality of these products (Smith et al., 2004).
The results of a study conducted by Sultan Dalal et al. on microbial contamination of fresh sweets in south of Tehran indicated that 72.7% of the sweets in the study were contaminated with Enterobacteriaceae (40%), yeasts (33%) and Staphylococcus aureus (12%), which were the highest types of microbes detected (Soltan Dalal et al., 2010). Another study in Gorgan investigated the contamination of sweets containing cream. The results confirmed that 56% of the samples were infected with Enterobacteriaceae and 43.3% were infected with Staphylococcus aureus, and the highest contamination of sweets was in summer (Shabani et al., 2014).
The study of microbial quality control regarding sweet samples in Leeds, England, showed that 28% of the total samples were infected with Escherichia coli (Azhdari and Hashemzehi, 2020). In evaluation of sweets containing cream and ice creams, 50% of the samples were infected with Escherichia coli and 30% with Staphylococcus aureus (Khoramrooz et al., 2015). Due to the increase in food poisoning caused by the consumption of contaminated sweets in recent years, few studies were performed in this regard. The present study was conducted to investigate the microbial and fungal variety and load in Yazd traditional confectionery products such as Baqlava, Quttab, Pashmak, Nan Berenji, Loz and Hajibadam. These products have different ingredients and moisture levels, which are definitely effective on the type and level of contamination.
Materials and Methods
This cross-sectional study was conducted in 2020 in Yazd, and chemicals and culture media used in this study were prepared and applied according to the Iranian National Standard numbers 2395 and 9899 from the German company (Merck) (Institute of Standards, 2020). Sample preparation and dilutions were performed using the Iranian National Standard No. 8923-1 and 8923-4. The traditional sweet samples were taken from Yazd province confectioneries. One hundred and seventy samples received by Food, Cosmetics and Hygiene Supervision Department of the Food and Drug Administration of Yazd Shahid Sadoughi University of Medical Sciences were used in this study. The methods used in microbiological experiments were in accordance with the Institute of Standards and Industrial Research of Iran standards of 10899-3, 1810, 6806-3, 2964, 2461-1, 2461-2 (Khoramrooz et al., 2015, Soltan Dalal et al., 2010). Microbial tests including Enterobacteriaceae count, Escherichia coli count, Staphylococcus aureus count, Salmonella count, mold and yeast count were evaluated.
Samples included various Yazd traditional confectionery products which were investigated as soon as possible after entering the laboratory. Five grams of samples were mixed with 45 ml of ringer solution, and they were cultured using specific culture media (for each type of microorganism). They were incubated at different temperatures (each sample was incubated for a certain period according to the type of bacteria tested). The samples were taken out afterwards, and their contamination was determined by national standard values (Institute of Standards, 2020). Samples with hihger than standard contamination were declared unusable, and samples without contamination or the ones with less than standard contamination were accepted (Table 1).
Enterobacteriaceae were counted according to the Iranian national standard numbers, 2461-2 and 2461-1. Escherichia coli was examined in accordance with the standard number 2946. Staphylococcus aureus coagulase positive test was performed according to the Iranian National Standard Number 6806-3. Salmonella was also examined according to the standard number 1810. Mold and yeast tests were performed according to the number of 10899-3.
Data analysis: Data were analyzed using SPSS 22 software and Chi-square and Fisher tests.
| Table 1. Microbial tests and standard allowable limits for dry and semi-dry sweets. | ||
| Permissible limit to pollution | Type of pollution | |
| Semi-dry | Dry | |
| 102 | Negative | Enterobacteriaceae |
| Negative | Negative | Escherichia coli |
| Negative | Negative | Staphylococcus |
| Negative | Negative | Salmonella |
| 102 | 102 | Yeast |
| 102 | Negative | Mold |
Results
Analysis of the data showed that 8.83% of the total samples were infected with Escherichia coli, 17.06% with Enterobacteriaceae, 20.59% with mold, and 3.53% with yeast. Moreover, no infection was detected with Staphylococcus aureus and Salmonella. (Figure 1, Table 2)
Analysis of the data showed that 8.83% of the total samples were infected with Escherichia coli, 17.06% with Enterobacteriaceae, 20.59% with mold, and 3.53% with yeast. Moreover, no infection was detected with Staphylococcus aureus and Salmonella. (Figure 1, Table 2)
| Table 2. Distribution of microbial contamination in Yazd traditional sweets (n = 100). | |
| Result | Test name |
| The growth rate of bacteria in 91.17% of the samples were within the standard range and 8.83% were higher than the standard range. | E.coli confirmation test |
| The growth rate of bacteria in 82.94% of the samples were within the standard range and 17.06% were higher than the standard range. | Enterobacteriaceae confirmation test |
| The growth rate of bacteria in 100% of the samples was within the standard range | Staphylococcus aureus confirmation test |
| The growth rate of bacteria in 100% of the samples was within the standard range | Salmonella confirmation test |
| The growth rate of bacteria in 79.41% of the samples were within the standard range and 20.59% were higher than the standard range. | Mold confirmation test |
| The growth rate of bacteria in 96.47% of the samples were within the standard range and 3.53% were higher than the standard range. | Yeast confirmation test |
The collected samples were from different types of traditional sweets in Yazd province, and the highest level of microbial contamination was related to pistachio nuts, and the lowest level of contamination was attributed to traditional HajiBadam sweets. Examples of detected fungi and yeasts were presented in Figure 2.
Discussion
There are numerous reports of diseases and food poisoning due to microbial contamination of food in different parts of the world (Little and De Louvois, 1999) in developed countries. The incidence of these diseases and the number of cases or deaths are continuously recorded by health care centers. There is high frequency of food microbial poisoning In Iran (Jay, 1992). Many cases of gastroenteritis due to the consumption of contaminated food have been referred to hospitals and emergency centers every day. Most of them were due to the lack of performance of hygienic principles in preparing food or storing them (Karim et al., 1995). Fecal bacteria including coliforms and enterococci species are also one of the most important infections of food. These bacteria are important in food industry, and in recent years, they have been considered as the best indicator of fecal contamination (Wouafo et al., 1996). The results of the present study showed that 13.2% of the samples were infected with Enterobacteriaceae, which can be attributed to the people working in confectionery workshops. Contamination of traditional sweets with Enterobacteriaceae family can be due to the preparation of food in contaminated containers and the lack of personal hygiene by confectionery staff, which results in the spread of fecal bacteria during the production and distribution of sweets.
Since these bacteria are present in gastrointestinal tract, urinary tract, and water and soil, compliance with personal and environmental hygiene standards and the use of safe water can play an effective role in reducing this kind of pollution (Malekzadeh, 1995). Considering the importance of Escherichia coli as one of the factors involved in food poisoning, various studies were conducted in Iran regarding the contamination of sweets including sweet cream with coliform and Escherichia coli bacteria. for example, in a study conducted by Khezri et al. in Mashhad, the rate of contamination of sweets containing sweet cream with coliform and Escherichia coli was 69% and 26%, respectively (Khezri et al., 2007) . In Fars province, a study by Khalili Tehrani et al. showed that the rate of contamination of sweet creams used in confectioneries with Escherichia coli was high and 69% of creams were infected with this bacterium (Khalili et al., 2014). In the present study, only 5% of traditional pastry samples were infected with Escherichia coli. Because Escherichia coli was an indicator of fecal contamination, this opportunistic bacterium caused diarrhea in children and adults by producing enterotoxins. Epidemics of this bacterium were attributed to the consumption of contaminated water and food. This level of pollution indicated lack of personal hygiene and that the personnel did not use gloves in production, distribution, and transportation centers (Malekzadeh, 2003).
The results of Nikniaz’s study in Tabriz showed that 48.8% of creamy pastry samples were infected with Escherichia coli, 38.8% with coliforms, 31.2% with Staphylococcus aureus, and 70% were infected with yeasts (Nikniaz et al., 2011). In a study by Faramarzi et al. in the west of Tehran, it was found that out of 642 food samples, 4.81% of the sweets were infected with Staphylococcus aureus, and 4.81% with Bacillus cereus (Faramarzi et al., 2012). Hosseini's study showed that out of 216 samples of sweets containing sweet cream collected from Tehran, which were examined microbiologically, 83% were reported to be unusable and were an important cause of infection with Enterobacteriaceae (Hosseini, 1999). In Khezri's study, the rate of contamination of sweets containing sweet cream with coliform and Escherichia coli was 69% and 26% (Khezri et al., 2007).
In the present study, the rate of contamination of traditional sweets with mold and yeast was 20.59% and 3.53%. In the studies conducted in Mashhad, the contamination of sweets containing sweet cream with mold was 9%, and the contamination of sweets containing sweet cream with mold and yeast in Zahedan was 5.9% (Khezri et al., 2007, Shadan et al., 2003). The high level of contamination in the present study might be due to poor maintenance conditions. Contamination of traditional sweets with mold, in addition to causing health effects and reduced quality of product, is economically significant for confectionery industry (Smith et al., 2004). Because fungal spores are scattered in the air, they can contaminate sweets. In addition to air pollution, contamination of confectionery containers, distributors, and fungal contamination of confectionery raw materials such as sugar, flour, and especially nuts such as pistachio and almond nuts can also lead to mold contamination of traditional sweets (Caprioli et al., 1994). By observing hygiene, storing, and distributing confectionery products in a suitable and aseptic environment and using preservatives such as sorbates and benzoates, yeast contamination of this product can be reduced to some extent (Azhdari and Hashemzehi, 2020).
Lack of hygiene in confectionery and transmission of various microorganisms through contaminated food increases the acquired immunity of individuals against many of these microorganisms; but children, the elderly people with weakened immune systems and some other vulnerable people in the community are always more susceptible to diseases caused by these microorganisms. In addition to children and vulnerable groups, tourists are also more susceptible to infections and food poisoning due to the lack of previous exposure to some microbial strains in food and the lack of acquired resistance to these strains (Caprioli et al., 1994).
A high percentage of traditional sweets distributed in this study did not meet the standards; therefor, following the standards and performing hygienic procedures are very important. In this regard, in order to control primary pollution, improving the level of knowledge of manufacturers in the field of hygiene in all stages of production and distribution to reduce the occurrence of secondary pollution is one of the ways to increase the hygienic level of these products. Observance of these principles in traditional sweets of Yazd is of special importance and should be evaluated in different stages of production and distribution of these products.
Conclusions
The findings of the present study suggest the high prevalence of microbial and fungal contamination of traditional sweets in Yazd, which leads to an increase in the risk of poisoning and diseases transmitted by eating these sweets. Therefore, training experts for correct control of health issues and supervision in the stages of preparation, production, transportation, storage, and supply in order to prevent the transmission of microbial contamination is of great importance.
Acknowledgements
The authors would like to thank Dr Kaveh Kavousi, who read the original manuscript and offered invaluable comments, and Dr Somayeh Moosavi, the manager of food laboratory in Food And Drug Faculty of Shahid Sadughi University of Yazd for their support.
Conflict of interests
The authors declared no conflict of interests.
Authors’ contributions
Kimiaee M and Madani M designed and conducted the study; Madani M analyzed data; Kimiaee M wrote the manuscript; Seyed Moshtaghion SM, Mosaddegh MH and Madani M revised the manuscript; and Madani M supervised the study. All the authors read and approved the fnal manuscript
Funding
We have no funding in this study.
References
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Bolton D, Meally A, Blair I, McDowell D & Cowan C 2008. Food safety knowledge of head chefs and catering managers in Ireland. Food control. 19 (3): 291-300.
Bula-Rudas FJ, Rathore MH & Maraqa NF 2015. Salmonella infections in childhood. Advances in pediatrics. 62 (1): 29-58.
Caprioli A, et al. 1994. Communitywide outbreak of hemolytic-uremic syndrome associated with non-O157 verocytotoxin-producing Escherichia coli. Journal of infectious diseases. 169 (1): 208-211.
Faramarzi T, et al. 2012. A survey on Bacterial Contamination of Food Supply in the West of Tehran. Journal of Fasa University of Medical Sciences. 2 (1): 11-18.
Hosseini H 1999. The survey of microbical contamination in fresh pastry in Tehran during the summer 1998- 1999. In The First National Seminar in Food Hygiene: Tehran, Iran.
Institute of Standards 2020. Microbiologic characteristics of sweets products, Institute of Standards and Industrial Research of Iran, no 2395. In 3rd. Institute of Standards: Tehran, Iran.
Jay J 1992. Modern food microbiology. Chapman and Hall: New York:.
Karim G, Razavilar V & Akhondzadeh Basti A 1995. Survey on the contamination of traditional ice cream with food born infection and intoxication bacteria. Veterinary Faculty University of Tehran. 50 ((1-2)): 71-78.
Khalili T, Dehghanzade K, Talebzade Z & Moazi P 2014. Pastry cream is consumed in the E. coli contamination in Shiraz. . In Eighth Congress of Microbiology: Tehran, Iran.
Khezri M, Safamanesh S & Gorgani M 2007. The survey of microbial contamination in dried and creamy sweets Food and Drug Deputy of Mashhad University of Medical Sciences.
Khoramrooz S, et al. 2015. Microbial contamination determination of Cream suit, Traditional Ice Cream and Olovia in Yasuj City. Armaghane danesh. 20 (6): 526-537.
Little C & De Louvois J 1999. Health risks associated with unpasteurized goats' and ewes' milk on retail sale in England and Wales. A PHLS Dairy Products Working Group Study. Epidemiology & Infection. 122 (3): 403-408.
Malekzadeh F 1995. Microbiology. Tehran University Press: Tehran, Iran.
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Meng J & Doyle MP 2002. Introduction. Microbiological food safety. Microbes and Infection. 4 (4): 395-397.
Mozafari N, Forouhesh Tehrani H & Salek Moghadam A 2002. The prevalence of Enterobacteriaceae producing heat-stable enterotoxin in food sent to the microbiology laboratory of food science research and educational center lab. Journal of research in medical sciences. 26 (1): 65-69.
Newell DG, et al. 2010. Food-borne diseases—the challenges of 20 years ago still persist while new ones continue to emerge. International journal of food microbiology. 139: S3-S15.
Nikniaz Z, Mahdavi R, Jalilzadeh H & VahedJM 2011. Evaluation of microbial contamination in cream filled pastries distributed in Tabriz confectionaries. Iournal of food technology and nutrition. 8 (1): 66-71.
Ostadrahimi A 2010. Importance of water quality control in food safety, case study: drinking, dairy and caning industries of east Azerbaijan. Medical Journal of Tabriz University of Medical Sciences. 29 (1): 93-97.
Shabani S, Sadeghi Mahoonak A & Jalali H 2014. Microbial contamination of pastry cream supplied in Gorgan. Medical laboratory journal. 8 (2): 62-66.
Shadan M, Khoushabi F & Safari F 2003. The evaluation of physicochemical and microbial status of traditional ice creams in Zahedan. Zahedan journal of research in medical sciences. 4 (4): 215-222.
Smith JP, Daifas DP, El-Khoury W, Koukoutsis J & El-Khoury A 2004. Shelf life and safety concerns of bakery products—a review. Critical reviews in food science and nutrition. 44 (1): 19-55.
Soltan Dalal M, Fazelifard P, Tabatabai Befroee A, Rashidi S & Zarrin M 2010. Determination of microbial contamination of fresh pastries supplies units in southern Tehran. Scientific journal of microbial biotechnology. 2: 7-11.
Wouafo M, Njine T & Tailliez R 1996. Hygiene and microbiologic quality of ice creams produced in Cameroon. A public health problem. Bulletin de la Societe de pathologie exotique. 89 (5): 358-362.
There are numerous reports of diseases and food poisoning due to microbial contamination of food in different parts of the world (Little and De Louvois, 1999) in developed countries. The incidence of these diseases and the number of cases or deaths are continuously recorded by health care centers. There is high frequency of food microbial poisoning In Iran (Jay, 1992). Many cases of gastroenteritis due to the consumption of contaminated food have been referred to hospitals and emergency centers every day. Most of them were due to the lack of performance of hygienic principles in preparing food or storing them (Karim et al., 1995). Fecal bacteria including coliforms and enterococci species are also one of the most important infections of food. These bacteria are important in food industry, and in recent years, they have been considered as the best indicator of fecal contamination (Wouafo et al., 1996). The results of the present study showed that 13.2% of the samples were infected with Enterobacteriaceae, which can be attributed to the people working in confectionery workshops. Contamination of traditional sweets with Enterobacteriaceae family can be due to the preparation of food in contaminated containers and the lack of personal hygiene by confectionery staff, which results in the spread of fecal bacteria during the production and distribution of sweets.
Since these bacteria are present in gastrointestinal tract, urinary tract, and water and soil, compliance with personal and environmental hygiene standards and the use of safe water can play an effective role in reducing this kind of pollution (Malekzadeh, 1995). Considering the importance of Escherichia coli as one of the factors involved in food poisoning, various studies were conducted in Iran regarding the contamination of sweets including sweet cream with coliform and Escherichia coli bacteria. for example, in a study conducted by Khezri et al. in Mashhad, the rate of contamination of sweets containing sweet cream with coliform and Escherichia coli was 69% and 26%, respectively (Khezri et al., 2007) . In Fars province, a study by Khalili Tehrani et al. showed that the rate of contamination of sweet creams used in confectioneries with Escherichia coli was high and 69% of creams were infected with this bacterium (Khalili et al., 2014). In the present study, only 5% of traditional pastry samples were infected with Escherichia coli. Because Escherichia coli was an indicator of fecal contamination, this opportunistic bacterium caused diarrhea in children and adults by producing enterotoxins. Epidemics of this bacterium were attributed to the consumption of contaminated water and food. This level of pollution indicated lack of personal hygiene and that the personnel did not use gloves in production, distribution, and transportation centers (Malekzadeh, 2003).
The results of Nikniaz’s study in Tabriz showed that 48.8% of creamy pastry samples were infected with Escherichia coli, 38.8% with coliforms, 31.2% with Staphylococcus aureus, and 70% were infected with yeasts (Nikniaz et al., 2011). In a study by Faramarzi et al. in the west of Tehran, it was found that out of 642 food samples, 4.81% of the sweets were infected with Staphylococcus aureus, and 4.81% with Bacillus cereus (Faramarzi et al., 2012). Hosseini's study showed that out of 216 samples of sweets containing sweet cream collected from Tehran, which were examined microbiologically, 83% were reported to be unusable and were an important cause of infection with Enterobacteriaceae (Hosseini, 1999). In Khezri's study, the rate of contamination of sweets containing sweet cream with coliform and Escherichia coli was 69% and 26% (Khezri et al., 2007).
In the present study, the rate of contamination of traditional sweets with mold and yeast was 20.59% and 3.53%. In the studies conducted in Mashhad, the contamination of sweets containing sweet cream with mold was 9%, and the contamination of sweets containing sweet cream with mold and yeast in Zahedan was 5.9% (Khezri et al., 2007, Shadan et al., 2003). The high level of contamination in the present study might be due to poor maintenance conditions. Contamination of traditional sweets with mold, in addition to causing health effects and reduced quality of product, is economically significant for confectionery industry (Smith et al., 2004). Because fungal spores are scattered in the air, they can contaminate sweets. In addition to air pollution, contamination of confectionery containers, distributors, and fungal contamination of confectionery raw materials such as sugar, flour, and especially nuts such as pistachio and almond nuts can also lead to mold contamination of traditional sweets (Caprioli et al., 1994). By observing hygiene, storing, and distributing confectionery products in a suitable and aseptic environment and using preservatives such as sorbates and benzoates, yeast contamination of this product can be reduced to some extent (Azhdari and Hashemzehi, 2020).
Lack of hygiene in confectionery and transmission of various microorganisms through contaminated food increases the acquired immunity of individuals against many of these microorganisms; but children, the elderly people with weakened immune systems and some other vulnerable people in the community are always more susceptible to diseases caused by these microorganisms. In addition to children and vulnerable groups, tourists are also more susceptible to infections and food poisoning due to the lack of previous exposure to some microbial strains in food and the lack of acquired resistance to these strains (Caprioli et al., 1994).
A high percentage of traditional sweets distributed in this study did not meet the standards; therefor, following the standards and performing hygienic procedures are very important. In this regard, in order to control primary pollution, improving the level of knowledge of manufacturers in the field of hygiene in all stages of production and distribution to reduce the occurrence of secondary pollution is one of the ways to increase the hygienic level of these products. Observance of these principles in traditional sweets of Yazd is of special importance and should be evaluated in different stages of production and distribution of these products.
Conclusions
The findings of the present study suggest the high prevalence of microbial and fungal contamination of traditional sweets in Yazd, which leads to an increase in the risk of poisoning and diseases transmitted by eating these sweets. Therefore, training experts for correct control of health issues and supervision in the stages of preparation, production, transportation, storage, and supply in order to prevent the transmission of microbial contamination is of great importance.
Acknowledgements
The authors would like to thank Dr Kaveh Kavousi, who read the original manuscript and offered invaluable comments, and Dr Somayeh Moosavi, the manager of food laboratory in Food And Drug Faculty of Shahid Sadughi University of Yazd for their support.
Conflict of interests
The authors declared no conflict of interests.
Authors’ contributions
Kimiaee M and Madani M designed and conducted the study; Madani M analyzed data; Kimiaee M wrote the manuscript; Seyed Moshtaghion SM, Mosaddegh MH and Madani M revised the manuscript; and Madani M supervised the study. All the authors read and approved the fnal manuscript
Funding
We have no funding in this study.
References
Azhdari A & Hashemzehi N 2020. Evaluation of microbial quality of dry confectioneries, marketed in Birjand city. Journal of food microbiology. 7 (3): 13-22.
Bolton D, Meally A, Blair I, McDowell D & Cowan C 2008. Food safety knowledge of head chefs and catering managers in Ireland. Food control. 19 (3): 291-300.
Bula-Rudas FJ, Rathore MH & Maraqa NF 2015. Salmonella infections in childhood. Advances in pediatrics. 62 (1): 29-58.
Caprioli A, et al. 1994. Communitywide outbreak of hemolytic-uremic syndrome associated with non-O157 verocytotoxin-producing Escherichia coli. Journal of infectious diseases. 169 (1): 208-211.
Faramarzi T, et al. 2012. A survey on Bacterial Contamination of Food Supply in the West of Tehran. Journal of Fasa University of Medical Sciences. 2 (1): 11-18.
Hosseini H 1999. The survey of microbical contamination in fresh pastry in Tehran during the summer 1998- 1999. In The First National Seminar in Food Hygiene: Tehran, Iran.
Institute of Standards 2020. Microbiologic characteristics of sweets products, Institute of Standards and Industrial Research of Iran, no 2395. In 3rd. Institute of Standards: Tehran, Iran.
Jay J 1992. Modern food microbiology. Chapman and Hall: New York:.
Karim G, Razavilar V & Akhondzadeh Basti A 1995. Survey on the contamination of traditional ice cream with food born infection and intoxication bacteria. Veterinary Faculty University of Tehran. 50 ((1-2)): 71-78.
Khalili T, Dehghanzade K, Talebzade Z & Moazi P 2014. Pastry cream is consumed in the E. coli contamination in Shiraz. . In Eighth Congress of Microbiology: Tehran, Iran.
Khezri M, Safamanesh S & Gorgani M 2007. The survey of microbial contamination in dried and creamy sweets Food and Drug Deputy of Mashhad University of Medical Sciences.
Khoramrooz S, et al. 2015. Microbial contamination determination of Cream suit, Traditional Ice Cream and Olovia in Yasuj City. Armaghane danesh. 20 (6): 526-537.
Little C & De Louvois J 1999. Health risks associated with unpasteurized goats' and ewes' milk on retail sale in England and Wales. A PHLS Dairy Products Working Group Study. Epidemiology & Infection. 122 (3): 403-408.
Malekzadeh F 1995. Microbiology. Tehran University Press: Tehran, Iran.
Malekzadeh F 2003. Microbiology. Tehran University Press: Tehran, Iran.
Meng J & Doyle MP 2002. Introduction. Microbiological food safety. Microbes and Infection. 4 (4): 395-397.
Mozafari N, Forouhesh Tehrani H & Salek Moghadam A 2002. The prevalence of Enterobacteriaceae producing heat-stable enterotoxin in food sent to the microbiology laboratory of food science research and educational center lab. Journal of research in medical sciences. 26 (1): 65-69.
Newell DG, et al. 2010. Food-borne diseases—the challenges of 20 years ago still persist while new ones continue to emerge. International journal of food microbiology. 139: S3-S15.
Nikniaz Z, Mahdavi R, Jalilzadeh H & VahedJM 2011. Evaluation of microbial contamination in cream filled pastries distributed in Tabriz confectionaries. Iournal of food technology and nutrition. 8 (1): 66-71.
Ostadrahimi A 2010. Importance of water quality control in food safety, case study: drinking, dairy and caning industries of east Azerbaijan. Medical Journal of Tabriz University of Medical Sciences. 29 (1): 93-97.
Shabani S, Sadeghi Mahoonak A & Jalali H 2014. Microbial contamination of pastry cream supplied in Gorgan. Medical laboratory journal. 8 (2): 62-66.
Shadan M, Khoushabi F & Safari F 2003. The evaluation of physicochemical and microbial status of traditional ice creams in Zahedan. Zahedan journal of research in medical sciences. 4 (4): 215-222.
Smith JP, Daifas DP, El-Khoury W, Koukoutsis J & El-Khoury A 2004. Shelf life and safety concerns of bakery products—a review. Critical reviews in food science and nutrition. 44 (1): 19-55.
Soltan Dalal M, Fazelifard P, Tabatabai Befroee A, Rashidi S & Zarrin M 2010. Determination of microbial contamination of fresh pastries supplies units in southern Tehran. Scientific journal of microbial biotechnology. 2: 7-11.
Wouafo M, Njine T & Tailliez R 1996. Hygiene and microbiologic quality of ice creams produced in Cameroon. A public health problem. Bulletin de la Societe de pathologie exotique. 89 (5): 358-362.
Type of article: orginal article |
Subject:
public specific
Received: 2022/07/18 | Published: 2024/05/21 | ePublished: 2024/05/21
Received: 2022/07/18 | Published: 2024/05/21 | ePublished: 2024/05/21
References
1. Azhdari A & Hashemzehi N 2020. Evaluation of microbial quality of dry confectioneries, marketed in Birjand city. Journal of food microbiology. 7 (3): 13-22.
2. Bolton D, Meally A, Blair I, McDowell D & Cowan C 2008. Food safety knowledge of head chefs and catering managers in Ireland. Food control. 19 (3): 291-300.
3. Bula-Rudas FJ, Rathore MH & Maraqa NF 2015. Salmonella infections in childhood. Advances in pediatrics. 62 (1): 29-58.
4. Caprioli A, et al. 1994. Communitywide outbreak of hemolytic-uremic syndrome associated with non-O157 verocytotoxin-producing Escherichia coli. Journal of infectious diseases. 169 (1): 208-211.
5. Faramarzi T, et al. 2012. A survey on Bacterial Contamination of Food Supply in the West of Tehran. Journal of Fasa University of Medical Sciences. 2 (1): 11-18.
6. Hosseini H 1999. The survey of microbical contamination in fresh pastry in Tehran during the summer 1998- 1999. In The First National Seminar in Food Hygiene: Tehran, Iran.
7. Institute of Standards 2020. Microbiologic characteristics of sweets products, Institute of Standards and Industrial Research of Iran, no 2395. In 3rd. Institute of Standards: Tehran, Iran.
8. Jay J 1992. Modern food microbiology. Chapman and Hall: New York:.
9. Karim G, Razavilar V & Akhondzadeh Basti A 1995. Survey on the contamination of traditional ice cream with food born infection and intoxication bacteria. Veterinary Faculty University of Tehran. 50 ((1-2)): 71-78.
10. Khalili T, Dehghanzade K, Talebzade Z & Moazi P 2014. Pastry cream is consumed in the E. coli contamination in Shiraz. . In Eighth Congress of Microbiology: Tehran, Iran.
11. Khezri M, Safamanesh S & Gorgani M 2007. The survey of microbial contamination in dried and creamy sweets Food and Drug Deputy of Mashhad University of Medical Sciences.
12. Khoramrooz S, et al. 2015. Microbial contamination determination of Cream suit, Traditional Ice Cream and Olovia in Yasuj City. Armaghane danesh. 20 (6): 526-537.
13. Little C & De Louvois J 1999. Health risks associated with unpasteurized goats' and ewes' milk on retail sale in England and Wales. A PHLS Dairy Products Working Group Study. Epidemiology & Infection. 122 (3): 403-408.
14. Malekzadeh F 1995. Microbiology. Tehran University Press: Tehran, Iran.
15. Malekzadeh F 2003. Microbiology. Tehran University Press: Tehran, Iran.
16. Meng J & Doyle MP 2002. Introduction. Microbiological food safety. Microbes and Infection. 4 (4): 395-397.
17. Mozafari N, Forouhesh Tehrani H & Salek Moghadam A 2002. The prevalence of Enterobacteriaceae producing heat-stable enterotoxin in food sent to the microbiology laboratory of food science research and educational center lab. Journal of research in medical sciences. 26 (1): 65-69.
18. Newell DG, et al. 2010. Food-borne diseases—the challenges of 20 years ago still persist while new ones continue to emerge. International journal of food microbiology. 139: S3-S15.
19. Nikniaz Z, Mahdavi R, Jalilzadeh H & Vahed JM 2011. Evaluation of microbial contamination in cream filled pastries distributed in Tabriz confectionaries. Iournal of food technology and nutrition. 8 (1): 66-71.
20. Ostadrahimi A 2010. Importance of water quality control in food safety, case study: drinking, dairy and caning industries of east Azerbaijan. Medical Journal of Tabriz University of Medical Sciences. 29 (1): 93-97.
21. Shabani S, Sadeghi Mahoonak A & Jalali H 2014. Microbial contamination of pastry cream supplied in Gorgan. Medical laboratory journal. 8 (2): 62-66.
22. Shadan M, Khoushabi F & Safari F 2003. The evaluation of physicochemical and microbial status of traditional ice creams in Zahedan. Zahedan journal of research in medical sciences. 4 (4): 215-222.
23. Smith JP, Daifas DP, El-Khoury W, Koukoutsis J & El-Khoury A 2004. Shelf life and safety concerns of bakery products—a review. Critical reviews in food science and nutrition. 44 (1): 19-55.
24. Soltan Dalal M, Fazelifard P, Tabatabai Befroee A, Rashidi S & Zarrin M 2010. Determination of microbial contamination of fresh pastries supplies units in southern Tehran. Scientific journal of microbial biotechnology. 2: 7-11.
25. Wouafo M, Njine T & Tailliez R 1996. Hygiene and microbiologic quality of ice creams produced in Cameroon. A public health problem. Bulletin de la Societe de pathologie exotique. 89 (5): 358-362.
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