en public specific orginal article <div style="text-align: justify;"><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Staphylococcus aureus (S. aureus)</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> is responsible for most cases of food poisoning all around the world. <i>These</i> carriers and manipulated foodstuffs are the main sources of bacteria transmission to ready-to-eat food. This study aims to determine the genetic affinity of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains isolated from cream pastries and workers^, nasal of Shiraz city confectioneries. <b>&nbsp;Methods:</b> 30 MRSA<i> </i>strains (7 nasal carriers, 23 food isolates) were selected from the bank of bacteria at Shiraz medical faculty. To determine the genetic affinity of the isolates, RAPD-PCR technique was performed using OLP6, OLP11, and OLP13 primers. </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RAPD-PCR</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> patterns were analyzed using the software GelJ. <b>Results:</b> By using primer OLP6 only 5 </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RAPD-PCR</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> patterns were produced from DNA ampliqons of creamy pastry isolates and were not enough to compare the genetic affinity of all the isolates. Based on 100% similarity, OLP13 primer produced 20 different patterns with some bands in the range of 1 to 11, and the OLP11 primer produced 22 patterns with some bands from 3 to 11 bands. At closely and possibly genetically related levels, the isolates are categorized into (13-15) and (1-5) clusters. In general, all the isolates are classified into human and food isolates. <b>Conclusions:</b> There was no genetic affinity of MDSA isolates regarding human and food samples; but, a high percentage of close genetic relationship between the isolates increases the possibility of bacteria transfer from humans to pastries and food poisoning</span></span></div> Methicillin-resistant Staphylococcus aureus, Genetic affinity, Nasal carriers, Cream Pastry, RAPD-PCR. 487 495 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-480-2&slc_lang=en&sid=1 Ali Asghar Masihi Nezhad No Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Mojtaba Boniadian boniadian@sku.ac.ir 0000-0001-5970-9469 Yes Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Mohammad Motamedifar 0000-0002-9704-7811 No Department of Bacteriology and Virology, School of Medicine HIV/AIDS Research Center, Research Institute for Health, Shiraz University of Medical Sciences Zahra Naziri 0000-0002-6755-1910 No Department of Pathobiology, School of Veterinary Medicine, Shiraz University.