en public specific orginal article <div style="text-align: justify;"><a name="_Hlk110505482"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">Background: </span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">There is a global threat of methicillin-resistant <i>Staphylococcus aureus (S. aureus or </i>MRSA), which has been regarded as a priority pathogen by the </span></span></span></a><a name="_Hlk113737904"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">world health organization (WHO)</span></span></span></a><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">. Livestock and its products are the sources of MRSA which can often occur in poor breeding conditions. The present study aims to investigate the prevalence of MRSA and the rate of antibiotic residue in pasteurized and raw farm milk</span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">. <b>Methods:</b> This was a cross-sectional study conducted from April to July 2020. 250 samples (200 samples of raw milk in farms around Tehran and 50 samples of pasteurized milk) were cultured to evaluate the occurrence of <i>S. aureus</i> and its antimicrobial susceptibility profile to 7 antimicrobial panels. Hansen Kit was used to monitor antibiotic residue in milk. <b>Results:</b> <a name="_Hlk110505401">63 <i>S. aureus </i>isolates (25.2%) were detected from 250 milk samples. Among 200 raw milk samples, 48 (24%) <i>S. aureus </i>isolates were detected and no strain of <i>S. aureus</i> was isolated from pasteurized milk. The highest rates of resistance belonged to ampicillin (95.8%)</a></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">, </span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">amoxicillin/clavulanic acid (87.5%), tetracycline (50%), and cefoxitin (45.8%). Moreover, 43 (17.2%) out of 250 milk samples had antibiotic residue in the antibiotic residue test using Danish Hansen kit. <b>Conclusion</b>: The present study indicates a high prevalence of subclinical <i>S. aureus</i> in dairy herds in Tehran, Iran. The milk contaminated with <i>S. aureus</i> and MRSA, posed a risk to public health owing to the presence of a phenotype resistant to very common antibiotics</span></span></span></div> Milk, Staphylococcus aureus, Methicillin, Anti-bacterial agents 207 213 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-499-2&slc_lang=en&sid=1 Mohammad Mehdi Soltan Dallal msoltandallal@gmail.com Yes Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran Zohreh Didar No Department of Food Science and Technology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran Mohammad Reza Mohammadi No Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Saeed Vahedi No Department of Anesthesiology, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran Ronak Bakhtiari No Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran Seyedeh Zohre Mirbagheri No Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran Hedroosha Molla Agha Mirzaei No Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran