en public specific orginal article <div style="text-align: justify;"><span style="font-size:11pt"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Background: </span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Leghemoglobin is an oxygen transporter and a hemoprotein found in nodule roots of nitrogen fixator plants from <i>Leguminosae</i> family. Leghemoglobin is produced in response to presence of bacteria belonging to <i>Rhizobia</i> family. Structurally, leghemoglobin is similar to hemoglobin and has a kind of heme iron structure.&nbsp; Study of leghemoglobin as a heme iron containing agent can open up a new way for treatment and natural prevention of iron deficiency anemia and substitution with mineral iron salts. <b>Methods:</b> Five different cultivars of alfalfa plants including Esfahani, Bammi, Yazdy, Nik shahri, and Hamedani were cultivated in gardening farm of the faculty of agriculture in Shahid Chamran University in a linear form. Cultivation was done with biological fertilizer containing <i>Sinorhizobium meliloti</i> for test groups and without fertilizer for sham groups. After 60 days, the samples were harvested, and the amount of leghemoglobin produced in nodules was quantified using LaRue and Child fluorometric method. <b>Results: </b>The results from five cultivars of alfalfa planted with biological fertilizer showed a significant increase in viscosity of leghemoglobin in nodules of the test group in comparison with sham group (<i>P</i><0.0001). Moreover, the high viscosity of leghemoglobin in nodules of Bammi cultivar indicated the capacity of this plant to produce leghemoglobin in Ahvaz. <b>Conclusion:</b> According to the findings of this study, Bammi cultivar of alfalfa is a good option for future studies and even production of high amounts of leghemoglobin in Khuzestan Province.&nbsp;&nbsp; </span></span></span></span></span></span></span></span></div> Leghemoglobin, Alfalfa, Iron, Anemia, Nodule 404 411 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-514-1&slc_lang=en&sid=1 Amir Siahpoosh 0000-0003-0144-7042 No Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahwaz, Iran Mohammad Reza Siahpoosh 0000-0001-9187-8211 No Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran Soroush Alaei Alaeisoroush@gmail.com 0000-0002-4332-8503 Yes Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran