en public specific orginal article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span style="font-family:"Arial","sans-serif"" lang="FA"></span></span></span></span></span></span><br> &nbsp;<span style="font-size:11pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Background:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Foodborne diseases are caused by eating contaminated food. <i>Yersinia</i> species are among the bacteria involved in food contamination, such as meat, poultry, vegetables, and milk. This study aims to investigate the prevalence and characteristics of <i>Yersinia enterocolitica</i> and <i>Yersinia pseudotuberculosis</i> from raw milk in Tehran.<b> Methods:</b> In this study, 360 samples of raw milk were collected from farms around Tehran and examined according to the FDA method. Then, 25 ml of milk samples were added to 225 ml of Peptone Sorbitol Bile Broth and kept for 10 days at 4 &deg;C for enrichment. After 10 days, 0.5 ml of the sample was added to 0.5 ml of 0.72% KOH solution and 0.54% NaCl. After 30 sec, it was cultured in the selected Cefsulodin-Irgasan-Novobiocin agar (CINagar) medium to remove the normal flora. The plates were stored for 48 h at 28 &deg;C. The suspected Bull&#39;s eye colonies were purified and phenotyping tests were carried out on the selected colony. The 20 E API kits were used for confirming identification.<b> Results: </b>From 360 raw milk samples, 4 <i>Yersinia</i> isolates (1.1%) including one <i>Y. pseudotuberculosis </i>(0.27%) and three <i>Y. enterocolitica</i> (0.83%) were isolated. In addition to <i>Yersinia,</i> other bacteria such as <i>Klebsiella,</i> </span></span></span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Serratia,</span></span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> <i>Citrobacter,</i> and Providencia were isolated from milk samples. <b>Conclusion:</b> The findings showed that clean tap water and healthy cattle in livestock can be effective in preventing Yersinia contamination. Lack of personal and environmental hygiene could cause food contamination by <i>Yersinia</i> and other intestinal bacteria leading to gastrointestinal infections.</span></span></span></span></span></span><br> &nbsp;</div> Yersinia enterocolitica, Yersinia pseudotuberculosis, Milk, Diarrhea 114 121 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-499-1&slc_lang=en&sid=1 Sara Sharifi Yazdi 0000-0001-9350-2947 Yes School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Behzad Mobasseri No Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Parisa Torabi Bonab 0000-0003-0155-9116 No Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran; Sina Sharifi Yazdi 0000-0001-6097-6950 No Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Zohre Mirbagheri 0000-0001-6494-9678 No Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Soltan Dallal msoltandallal@gmail.com 0000-0002-3421-3974 No Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran