en public specific orginal article <div style="text-align: justify;"><strong>Background:</strong> Reference measurements are used to screen for abnormal blood lipids. The problem is that these reference values obtained in one population cannot be effective for another population. This study aimed to determine the reference values for blood lipids profiles in the population aged 25-64 years in Yazd<span dir="RTL">.</span> <strong>Methods:</strong> This descriptive study was based on the data of&nbsp;<em> Yazd Health</em>&nbsp;Study (YaHS) on 3800 adults by cluster sampling. The data set included gender, age, total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), and&nbsp; high-density lipoprotein-cholesterol (HDL-C). The linear percentile regression model and the generalized additive model for location, scale, and shape (GAMLSS) were fitted to the data and the reference values were predicted according to the regression coefficients. R-3.0.1 software was used for data analysis. <strong>Results:</strong> Refrence values for TC, LDL-C, and HDL-C were 109.43-275.72, 45.58-177.70, and 29.95-62.22 mg/dl. The trend of TC, TG, and LDL-C levels increased with age in both genders, but the trend of HDL-C in men decreased with age and remained almost constant in women<span dir="RTL">.</span> <strong>Conclusion</strong><strong>:</strong> In this study, for the population of Yazd, reference values for blood lipids were different in both genders and age groups. Reference values for lipid profile increased in men and women with age.These findings can be used in both prevention and clinical decisions.</div> Cholesterol, Blood lipids, Reference values 374 382 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-389-1&slc_lang=en&sid=1 Hossein Fallahzadeh 0000-0001-6518-366x No Center for Healthcare Data Modeling, School of Public Pealth, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mahdieh Momayyezi mahdieh_momayyezi@yahoo.com 0000-0002-8205-3231 Yes Deptartment of Biostatistics and Epidemiology, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Masoud Mirzaei 0000-0001-6455-0747 No Center for Healthcare Data Modeling, School of Public Pealth, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.