en public specific orginal article <div style="text-align: justify;">Superheated water extraction (SWE), is a novel method, used to extract proteins or other bioactive component from agriculture products<strong>. Methods:</strong> In this study, the extract from quinoa-barley malt (in a proportion of 30:70) was treated by SWE method. The effect of extraction time (15, 30, and 45 min) on physicochemical and functional properties of the extract was determined at 120 &ordm;C. <strong>Results:</strong> The result showed that by increasing the extraction time the amount of protein increased. The maximum protein (%) was obtained in 45 min of SWE. The results revealed that increasing the extraction time had a significant effect on the turbidity and pH. In addition, increasing the extraction time had a positive effect on the functional properties. The highest protein isolate (PI) solubility was at 120 &deg;C. <strong>Conclusions:</strong> Although SWE method appears to be a useful extraction method for producing valuable materials from cereal and pseudocereal, this method has only been used at pre-commercial scale and more investigation is required to study the quantity, quality, and stability of the extracted valuable materials to scale it up for industrial means.</div> Quinoa, Barley malt, Extraction, Superheated water extraction 51 59 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-333-1&slc_lang=en&sid=1 Samireh Sabah Sabah 0000-0002-4376-0216 No Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Anoshe Sharifan a_sharifan2000@yahoo.com 0000-0002-0849-6472 Yes Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Afshin Akhonzadeh Basti 0000-0002-8532-5757 No Department of Food Hygiene, School of Veterinary Medicine, University of Tehran, Tehran, Iran Behrooz Jannat 0000-0001-9208-2709 No Halal Research Center Islamic Republic of Iran, Tehran, Iran Maryam TajAbadi Ebrahimi 0000-0003-2780-3447 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran