en public specific orginal article <div style="text-align: justify;">Aflatoxin is one of the most important topics of research in food contamination . Aflatoxin, a member of the Mycotoxin family, is a secondary metabolite of molds such as <em>Aspergillus Flavus</em> and <em>Aspergillus Parasiticus</em> . These fungi also pollute the crops during production and storage . In the case of the products&rsquo; contamination with spores in the desired temperature and humidity conditions, the hogs grow, fungus is produced, and poison is created . One of the most deadly toxins is Aflatoxin , which is known as Mycotoxin due to its considerable abundance in the nature, toxicity, and carcinogenicity . Several types of Aflatoxin, i.e., G1, G2, B2, B1 were identified, among which B1 Aflatoxins (AFB1) are most important . AFB1 is the most toxic compound in this group. The highest concentration of AFB1 is observed in food and animal feed . The concentration range of AFB1 varies in different food products; for example, in pistachios. According to the European Union, the acceptable AFB1 is 12 ppb (Parts per billion), but this amount may vary in different countries &nbsp;from 2 to 20 ppb . Among the 400 known Mycotoxins, AFB1 is considered as the most dangerous one to human health due to its severe effects of toxicity, carcinogenicity, fetal defects, and mutagenesis.<br> Among the most commonly used to detect this toxin high-performance liquid chromatography (HPLC) and immunohistochemistry columns are widely used for detection of Aflatoxin and other agricultural products in research and commercial areas .</div> Aflatoxin, Raman Technique, Toxin, Food 293 295 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-123-7&slc_lang=en&sid=1 Mehrnoosh Shirdeli 0000000233613941 No Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ameneh Marzban 0000-0002-8111-3319 No Department of Human Ecology, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Fatemeh Yaghoubi 0000000177992106 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Matthew Shirani 0000000218175453 No Department of Biological Sciences, Chandler-Gilbert Community College, Maricopa County, Arizona, United States of America. MaryamSadat Shahidi maryamshahidi13@yahoo.com 0000000190643015 Yes Department of Biochemistry, Sirjan University of Medical Sciences, Sirjan, Iran