en public specific review article <strong>Background: </strong>Low vitamin D (vit D) serum levels have been linked to various diseases such as multiple sclerosis, cardiovascular diseases, and diabetes. Maintenance of optimal vit D level should be supported by foods and supplements. Limited food sources of vit D made researchers focus on food fortification in recent years. <strong>Methods: </strong>A systematic review of randomized controlled trials reporting the impact of vit D food fortification on 25-hydroxy vitamin D [25(OH)D] level was conducted on PubMed, Scopus, ISI Web of Science and Google Scholar from inception up to August 2017. <strong>Results: </strong>Sixteen studies met the inclusion criteria and most of them (n =14) have represented the significant effect of food fortification on the improvement of vit D status of the participants. However, two studies failed to find any reasonable associations. Furthermore, it was observed that vit D food fortification has a greater impact on the people with lower 25(OH)D serum levels. <strong>Conclusions: </strong>Fortification of foods with vit D has a significant impact on serum 25(OH)D concentrations. Therefore, food enrichment is an economical and effective approach for different populations to prevent vit D deficiency. Vitamin D, 25-hydroxy vitamin D, 1, 25 dihydroxy vitamin D, Food fortification. 126 141 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-92-3&slc_lang=en&sid=1 Roya Sakhaei https://orcid.org/0000-0002-6332-5115 Yes Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Nasir Talenezhad https://orcid.org/0000-0001-5317-4942 No Department of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohammad Mohammadi https://orcid.org/0000-0001-6397-4409 No Department of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Nahid Ramezani-Jolfaie https://orcid.org/0000-0002-2233-8665 No Department of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran