en public specific orginal article <div style="text-align: justify;"><span style="font-size:10.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">Vitamin D deficiency is common among women, particularly those who are overweight or obese. There is evidence that physical activity benefits on body vitamin D status. Therefore, this study assessed the effect of CrossFit training on serum 25(OH)D₃ levels and anthropometric measures in women. <b>Methods:</b> This randomized clinical trial enrolled 28 overweight/obese adult women. Participants were randomly allocated to a CrossFit (n=14) or a non-intervention group (n=14) for 8 weeks. Participants were then asked to complete CrossFit training for 1 hour, 3 times per week. Fasting levels of 25(OH)D₃ and anthropometric measurements, including weight, height, body mass index (BMI), waist-to-hip ratio (WHR), lean body mass (LBM), and body fat mass (BFM), were measured for each participant at the beginning and end of the study. <b>Results:</b> In total, 24 women (12 participants in the CrossFit and 12 participants in the non-intervention group) completed the trial. After 8 weeks of intervention, it was found a significant increase in serum 25(OH)D3 levels in the CrossFit group compared with other group (1.11&plusmn;0.45 ng/ml in the CrossFit group vs. 0.19&plusmn;0.44 ng/ml in the non-intervention group, <i>P</i><0.01). Furthermore, in the CrossFit group, significant decreases were found in body weight, BMI, and WHR and a significant increase in LBM, compared with the non-intervention group (<i>P</i>< 0.05).<b> Conclusion:</b> Findings indicate that 8 weeks of CrossFit training can improve vitamin D levels and anthropometric measures in overweight/obese adult women.</span></span></span></div> CrossFit training, TrialVitamin D, ObesityAnthropometric measures. 479 488 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-1705-1&slc_lang=en&sid=1 Fereshteh Dehghanpour Farashah fa.zd2010@gmail.com Yes Department of Physical Education, Taft Branch, Islamic Azad University, Taft, Yazd, Iran. Abdolmajid Emami Fa.zd2010@gmail.com No Department of Physical Education, Meybod Branch, Islamic Azad University, Meybod, Yazd, Iran.