en public specific orginal article <strong>Background:</strong><em> This study was conducted to determine the effects of </em>n-3 PUFAs <em>supplementation on plasma h</em>omocysteine<em> (Hcy) level, lipid profile and insulin resistance in patients with type 2 diabetes (T2D). </em><em><strong>Methods: </strong></em>This study is a double-blind controlled trial involving 70 patients with T2D selected from Yazd Diabetes Research Center in 2013<em>. Patients were randomly assigned to receive either 2 g/day omega-3 soft gels (OG) or 2 g/day placebo (PG) for 6 weeks. At the beginning and end of the study, Hcy concentration, fasting plasma glucose (FBG), fasting plasma insulin, total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c), high density lipoprotein cholesterol (HDL-c), HDL-c/LDL-c ratio, insulin resistance (HOMA-IR), insulin sensitivity (IS) and beta-cell function were measured and compared. </em><em><strong>Results: </strong></em>Sixty five participants completed the study. The results of this study showed that<br> omega-3 fatty acid supplementation caused significant increase in Hcy<br> (<em>P</em> = 0.007) and LDLc (<em>P</em> = 0.02), while HDLc and HDLc/LDLc ratio were significantly decreased (<em>P</em> = 0.001 and 0.006, respectively). In both groups, insulin and HOMA-IR were increased, while IS decreased significantly. &Beta;eta-cell function was increased only in OG (<em>P</em> = 0.005). There was no significant difference in mean change of any factors. <em><strong>Conclusion: </strong></em>The present study found no beneficial effects of <em>2 g/day omega-3 supplement for 6 weeks </em>on biomarkers of Hcy, FBG, insulin and lipid profile in <em>th T2D patients.</em> Type 2 diabetes, Homocysteine, Omega-3, Lipid profiles, HOMA-IR. 40 50 http://jnfs.ssu.ac.ir/browse.php?a_code=A-10-1-64&slc_lang=en&sid=1 Faezeh Poursoleiman No Department of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hassan Mozaffari-Khosravi Yes Does Omega-3 Fatty Acid Supplementation Have Beneficial Effects on Plasma Homocysteine, Insulin Resistance and Lipid Profile of Type 2 Diabetic Patients? A Randomized Clinical Trial Akram Naghdipour Biregani No Department of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran