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Volume 7, Issue 4 (NOV 2022)
JNFS 2022, 7(4): 508-511 |
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Rahnavard H. Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection. JNFS 2022; 7 (4) :508-511
URL: http://jnfs.ssu.ac.ir/article-1-413-en.html
URL: http://jnfs.ssu.ac.ir/article-1-413-en.html
School of Agricultural Sciences and Food Industries, Ardestan Branch of Islamic Azad University.
Abstract: (1610 Views)
Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Despite the optimal conditions, analytical property of the method containing lower limit of quantitation (2.00 ng g-1) and upper limit of quantitation (1000 ng g-1), linearity (r2=0.992), and recovery (103%) were acceptable. The results showed the range of 105 ± 0.09 - 875 ± 0.020 ng g-1. Conclusion: The method was successfully used to quantify the target analytes in all trout samples.
Keywords: Astaxanthin, High performance liquid chromatography, Fish, Upper limit of quantification (ULOQ), lower limit of quantification (LLOQ)
Type of article: orginal article |
Subject:
public specific
Received: 2021/05/20 | Published: 2022/11/19 | ePublished: 2022/11/19
Received: 2021/05/20 | Published: 2022/11/19 | ePublished: 2022/11/19
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